26: 343-345. Indian mustard (Brassica juncea L.Czern & Coss.) We also present a comparative analysis of nymphs and adult transcriptomes to study the differential expression profiles associated with different developmental stages as well as different feeding conditions. Image 1327096 is of mustard aphid (Lipaphis erysimi ) adult(s) on cabbage. Comparative efficacy of insecticides against mustard aphid, Lipaphis erysimi (Kalt.). Adult mustard aphid, Lipaphis erysimi. Thank you for your interest in spreading the word about bioRxiv. Blackman & Eastop list 25 species of aphid as feeding on Brassica species worldwide, and provide formal identification keys Adult mustard aphid, Lipaphis erysimi. 1977. Parasitized aphid (mummy) at bottom. The mustard aphid, Lipaphis erysimi Kaltenbach, is a major pest in mustard growing regions of the wo rld. Lipaphis erysimi is a Brassicaceae specialist aphid, which causes significant losses in yield and/or reduction of oil content of vegetable and oilseed brassicas and is a major pest in the Indian subcontinent. and their effect on Coccinella septumpunctata was also recorded at Pilibhit (UP) during 2007-08 on Indian mustard. Whilst we make every effort to ensure that identifications are correct, we cannot absolutely warranty their accuracy. Potential transcripts for host selection, detoxification, salivary proteins and effectors, molecular chaperones and developmental genes were identified. Turnip aphid, Mustard aphid. (Show World list). A total of 20441 found to share no homology to any sequence available in the public domain and could therefore represent novel aphid genes or sequences that are unique to L. erysimi. Distribution. Lipaphis erysimi is a Brassicaceae specialist aphid, which causes significant losses in yield and/or reduction of oil content of vegetable and oilseed brassicas and is a major pest in the Indian subcontinent. Large aphid populations can affect leaf size and yield. Plants remain stunted and sooty molds grow on the honey dew excreted by the insects. The functional response of fourth instars, adult males and females of the sevenspotted lady beetle, Coccinella septempunctata Linnaeus (Coleoptera: Coccinellidae), was studied at different densities of aphid, Lipaphis erysimi (Kaltenbach) (Homoptera: Aphididae). ), is a pest of cruciferous plants throughout the world but no information is available on its metabolic requirements or the composition of the honeydew. Pacific Pests and Pathogens - Fact Sheets Control of mustard aphid, Lipaphis erysimi (Kalt.) = 1 × 109 spores/ml) at 3 ml/l water, Agri Sakti (Beauveria bassiana a.i. Short Communication. Intr oduction Mustard crop attacked by painted bug, mustard Saw fly, mustard aphid potato aphid, leaf miner, flea beetle, diamond-back moth, bihar hairy catterpiller, cabbage buter fly and tobacco caterpillar Among them, Mustard aphid. crop in tropical and sub-tropical climate.Cultivated Brassica germplasm has failed so far to provide any source of resistance. Photo 3. This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. The swollen mustard aphids, Lipaphis erysimi, have been parasitised. Of those aphid species, Baker (2015) lists 12 as occurring in Britain The losses in yield by mustard aphid were 66-96% (Singh and Sachan, 1997). 2004, Carter and Sorensen 2013, Adhab and Schoelz 2015). They are known as "mummies". Materials and Methods ‘Sarson”, B. rapa cv. = 1 × 109 spores/ml) at 3.3 ml/l water, Varunastra (Verticillium lecanii spores 2% aqueous suspension, 2 × 108 CFU/ml) at 6 ml/l water, Mahastra (Bacillus thuringiensis var. with combination of … Chemical control of mustard aphid with newer and conventional insecticides. This is the subject of investigation. Despite the lateness of the year, there was no sign of any sexual forms and the aphids were reproducing viviparously (see picture below). 66, No. The aptera (see first picture below) has two rows of dark bands on the thorax and abdomen which unite into a single band near the tip of the abdomen. It is the most destructive of all the pests of mustard in Bangladesh (Alam et al., 1964; Ahmed et al., 1977; Haque & Miah, 1979) and reduces the yield of mustard considerably. Modelling regional level spatial distribution of aphid (Lipaphis erysimi) growth in Indian mustard using satellite-based remote sensing data. Dilawari, V.K., and Atwal, A.S. 1989. The cabbage aphid, Brevicoryne brassicae L. was also found in very small numbers. 2004; 66(2):30-32. The body length of Lipaphis erysimi alatae is 1.4-2.2 mm. 2010-2016b; 18(2):333-335. Biocide Manic (Metarhizium anisopliae a.i. For assistance on the terms used for aphid morphology we suggest the figure provided by Blackman & Eastop (2006). The body length of adult Lipaphis erysimi apterae is 1.4-2.4 mm. In the present study, RiD was highly upregulated in R. indica during aphid infestation initiating a defense system mediated by jasmonic acid (JA), but not by salicylic acid (SA)/abscisic acid (ABA). This study reports the de novo transcriptome of L. erysimi for the first time. For this, RNA-seq was performed on three different biological samples adults, nymphs (with all nymph stages pooled) and adults starved for 3 hours (referred to as Adult Feeding, AF; Nymphs Feeding, NF, and Adult Starved_3 hr, ANF samples henceforth). Second image: Lipaphis erysimi alate, copyright Alan Outen & Rothamsted Research, all rights reserved. Its efficacy as a tool of biological control of mustard aphid, Lipaphis erysimi was tested under field conditions for two consecutive years. Efficacy of newer insecticides was studied against mustard aphid, Lipaphis erysimi (Kalt.) It feeds on various different Brassicaceae, including Brassica, Raphanus, and Sinapis spp., although it is not usually found on field Brassica crops. We fully acknowledge these authors as the source for the (summarized) taxonomic information we have presented. Both nymphs and adults suck the sap from leaves, buds and pods. @inproceedings{Mishra2003BioEcologyAM, title={Bio-Ecology And Management Of Mustard Aphid, Lipaphis Erysimi (Kali.) Keeping the lack of information on the efficacy of this fungus on this particular aphid species, it was proposed to study the management aspects of mustard aphid, Lipaphis erysimi by use of different dosages of this fungus. Aphids (Lipaphis erysimi Kaltenback) often reduce mustard (Brassica juncea L.) yield in India.This study was conducted to determine if intercropping with aromatic plant species could provide an environmentally safe method for aphid control. Singh PK, 2006. The wild crucifer aphid does not host alternate. The insecticides used are acetamiprid 20 SP (125 g/ha) and imidachloprid 17.8 SL (150 ml/ha). Lipaphis pseudobrassicae in which that combined length is usually more than 2.4 times as long as the siphunculus). We have used the keys and species accounts of Blackman & Eastop (1994) and Blackman & Eastop (2006) supplemented with Blackman (1974), Stroyan (1977), Stroyan (1984), Blackman & Eastop (1984), Heie (1980-1995), Dixon & Thieme (2007) and Blackman (2010). The siphunculi are paler than the cauda and have dark tips. Characterization of α-amylase and its activity from mustard aphid Lipaphis erysimi (Kalt.) Resistance to mustard aphid infestation and its correlation to some physiological and biochemical traits in selected varieties of different Brassica species were studied. Curling may occur in infested leaves and at advanced stage plants may wither and die. A total of 23,532 transcripts that remained unannotated were subjected to BLAST against aphid sequences available at AphidBase and a total of 3091 transcripts had hits with sequences of other aphids in the database, out of which 1380 had protein hits. Males have been found in some countries. The antennal terminal process is 0.8-1.15 times times the length of the siphunculi (cf. The clarified slide mounts below are of adult viviparous female Lipaphis erysimi : wingless, and winged. Turnip aphid adults and immatures. The mustard aphid, Lipaphis erysimi (Kalt.) The peak populations (42.7) and (28.7) per leaf on Rainbow and Oscar varieties were recorded. RH . MATERIALS AND METHODS The aphids were cultured on greenhouse grown radish plants. Chemical Control of Mustard Aphid, Lipaphis erysimi Kalt and Leaf Webber, Crocidolomia binotalis Zell on Cabbage. Blackman & Eastop list 15 species of aphid as feeding on those 3 species, and all Sinapis worldwide, and provide formal identification keys Lipaphis erysimi. Then in mid-October 2018 we found colonies of wild crucifer aphid on seed heads of hedge mustard (Sisymbrium officinale, see picture below). This could also allow identification of novel target genes for development of RNAi-based aphid control methods. This study reports the de novo transcriptome of L. erysimi for the first time. was the most abundant species on the crop. Photo 2. Lipaphis pseudobrassicae is a cosmopolitan and somewhat polyphagous aphid with preference to plant species in the mustard family. Adult apterae of Lipaphis erysimi are small to medium sized yellowish green, grey green or olive green aphids, with a faint white wax bloom. is one of the most damaging of biotic stresses that confront this crop. Mustard aphid (Lipaphis erysimi) and English grain aphid (Sitobion avenae) are among the most important pests in mustard and wheat fields in Nepal. The studies on the relative occurrence and abundance of mustard aphid, Lipaphis erysimi (Kalt.) Imidacloprid application, as a seed coating agent on oilseed rape, is recommended to control mustard aphid, Lipaphis erysimi (Kaltenbach) (Hemiptera: Aphididae). J. Insect Sci. Abstract. It is by Alton N. Sparks, Jr. at University of Georgia. Mustard aphid: Lipaphis erysimi: Symptoms of damage. Adult winged mustard aphid, Lipaphis erysimi. We have mostly made identifications from high resolution photos of living specimens, along with host plant identity. Except where otherwise specified, all text and images on this page are copyright InfluentialPoints under a Creative Commons Attribution 3.0 Unported License on condition that a link is provided to InfluentialPoints.com, Creative Commons Attribution 3.0 Unported License. (Show British list). Then in mid-October 2018 we found colonies of wild crucifer aphid on seed heads of hedge mustard (Sisymbrium officinale, see picture below). Department of Botany, University of Delhi, Department of Botany, Deshbandhu College, University of Delhi, De novo transcriptome profiling of mustard aphid (Lipaphis erysimi) and differential expression of transcripts associated with developmental stages, feeding and non-feeding conditions. These mixed-species colonies of aphids on the seedheads of shepherd's purse were still present up till late November. Wild germplasm is a potential source of resistance against many threatening herbivores. Note the green bands on the back of the aphid. This aphid is globally distributed, located in all … Photo 2. Of those aphid species, Baker (2015) lists 21 as occurring in Britain Krishnaiah, K. and N. Jagan Mohan. The infected filed looks sickly and blighted in appearance. It lives on the undersides of leaves as well as on inflorescences, young shoots and growing points. On Mustard With Special Reference … A total of 27,112 transcripts were annotated with insect proteins from SwissProt, of which 4128 transcripts were components of 165 KEGG pathways. (Show British list). It is highly host specific, feeding exclusively on Brassica phloem sap. kurstaki 0.5% wettable powder) at 6 g/l water, Neemraj Super (Az… Any errors in identification or information are ours alone, and we would be very grateful for any corrections. is an important winter oilseeds crop of India.Mustard aphid, Lipaphis erysimi (Kalt.) Adult winged mustard aphid, Lipaphis erysimi. Alatae (see second picture above) have a black head and thorax and a dusky green abdomen with black bands near the tip and conspicuous dark marginal sclerites. Blackman & Eastop list 18 species of aphid as feeding on those 2 species, and all Raphanus worldwide, and provide formal identification keys Common names. In the great majority of cases, identifications have been confirmed by microscopic examination of preserved specimens. Lipaphis alliariae which has the antennal terminal process 1.2-1.35 the length of the siphunculi). (Show World list). Several of the colonies were comprised mainly of immatures (see picture below). Winged aphid in middle. A total of 17,296 transcripts were classified based on their Gene Ontology. L. erysimi is a serious pest of mustard in India. 6. The mustard aphid, Lipaphis erysimi (Kalt. They are known as 'mummies'. to allylisothiocyanate. The mustard or turnip aphid, Lipaphis erysimi pseudobrassicae (Davis), is one of the most destructive pests of brassica causing over 50% yield loss (Ronquist and Ahman 1990, Patel et al. The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. Response of mustard aphid, Lipaphis erysimi (Kalt.) Pesticides. Symptoms of damage: Both nymphs and adults suck the sap from leaves, buds and pods; Curling may occur in infested leaves and at advanced stage plants may wither and die; Plants remain stunted and sooty molds grow on the honey dew excreted by the insects; The infected field looks sickly and blighted in appearance Mustard aphid, Lipaphis erysimi (Kaltenbach) is a major constraint in increasing the yield of rapeseed-mustard crops in India. Enter multiple addresses on separate lines or separate them with commas. The swollen mustard aphids, Lipaphis erysimi, have been parasitised. The combined length of antennal segment III and the terminal process is usually less than 2.4 times the length of the siphunculus (cf. International … This is an exploratory study with no biological replicates. These however were mixed species colonies comprised of Lipaphis erysimi, Aphis fabae and Myzus persicae (see picture below). Lipaphis erysimi is usually regarded as a relatively common aphid in Britain, but until recently we have found it to be rather scarce in southern England. However, the significant repertoire of feeding- and development-related genes and their differential expression profiles generated in this study adds to the limited data available on L. erysimi and it would facilitate studies on the molecular basis of aphid feeding and development. revealed that aphid appeared on leaves during 3rd week of January and on the inflorescences during 2nd week of February and continued up to harvesting on both the varieties. (2020). Annals of Plant Protection Sciences. 2:103-108. Keywords: Mustard aphid, Lipaphis erysimi, identification, life cycle, host crop and management 1. Introduction. 2, pp. has been found to be great hindrance and cause severe reduction to rapeseed and mustard. Note the green bands on the back of the aphid. International Journal of Pest Management: Vol. (Show World list). is very devastating. Our particular thanks to Roger Blackman for images of his clarified slide mounts. NOTE: Your email address is requested solely to identify you as the sender of this article. Rapeseed and mustard are one of the most important oilseed crops in Jammu and Kashmir as they rank second to cereals. Of those aphid species, Baker (2015) lists 15 as occurring in Britain (Hemiptera) and its inhibition by Rorippa indica defensin. Mustard aphid, Lipaphis erysimi (L.) Kaltenbach is a perpetual annual threat in the cultivation of rapeseed- mustard (Brassica spp.) Kawada, K. and T. Murai. Entomopathogenic fungi, Verticillium lecani has been considered to be pathogenic to a wide range of insect-pests including aphids, thrips and white fly (Brownbridge, 1995; Hall and Burges, 1979; Hall, 1982; Heyler, Gill, Baywater and Chambers, 992). Then in late October we found much bigger colonies of Lipaphis erysimi on seedheads of shepherd's purse (Capsella bursa-pastoris). Indian J Ent. Mustard (Brassica) is the principal and popular oilseed crop in Bangladesh.This crop is infested by several species of insect pests of which the mustard aphid, Lipaphis erysimi (Kalt.) (Show British list). We especially thank Alan Outen, Bedfordshire Invertebrate Group, and Chris Shortall, Rothamsted Research, for permission to reproduce Alan's photo of a Lipaphis erysimi alate and for information about the colony. Photo 3. 99-105. Common Name. Micrographs of clarified mounts by permission of Roger Blackman, copyright AWP all rights reserved. Mustard aphid: Lipaphis erysimi. Lipaphis erysimi is usually regarded as a relatively common aphid in Britain, but until recently we have found it to be rather scarce in southern England. RiD was found to have an effective insecticidal property against mustard aphid, Lipaphis erysimi. 1979. Lipaphis erysimi is confined to Britain and northern continental Europe. Rohilla HR, Bhatnagar P, Yadav PR. (2008). Incidence of aphid during re productive stage of m ustard causes severe Entomologia experimentalis et applicata. Determination of the amount of honeydew secreted by aphids in a specific crop variety is one of the best ways to assess the susceptibility of aphid infestation to the variety. The more feeding of plant sap by aphid, the more secretion of honeydew. 15(2): 29-32. BSH 1 and Indian mustard, Brassica juncea cv. INTRODUCTION. Mustard aphid, Lipaphis erysimi (Kalt.) 4. A final transcriptome comprising 52,652 transcripts of 1064bp average length and N50 value of 1806 bp was generated. Great majority of cases, identifications have been parasitised Kali. ) this article erysimi is major... Was also found in very small numbers preprint is the author/funder, who has bioRxiv! Plants remain stunted and sooty molds grow on the undersides of leaves as well as on inflorescences, young and! Aphids were cultured on greenhouse grown radish plants with combination of … the mustard aphid, Lipaphis erysimi seedheads. On separate lines or separate them with commas along with host plant identity to identify you as sender. Sooty molds grow on the relative occurrence and abundance of mustard aphid, Lipaphis on. And effectors, molecular chaperones and developmental genes were identified information are ours alone, and Atwal A.S.. In Britain ( Show British list ) the cultivation of rapeseed- mustard ( Brassica spp. ),! May occur in infested leaves and at advanced stage plants may wither and die ) at 6 g/l,. Reduction to rapeseed and mustard are one of the aphid conventional insecticides indica. The word about bioRxiv were still present UP till late November the insects, the secretion! Sap from leaves, buds and pods Indian mustard using satellite-based remote sensing data were cultured greenhouse! We fully acknowledge these authors as the source for the first time British... The infected filed looks sickly and blighted in appearance in tropical and sub-tropical climate.Cultivated Brassica germplasm has so... Of leaves as well as on inflorescences, young shoots and growing points rapeseed and mustard are one of aphid! Is confined mustard aphid, lipaphis erysimi Britain and northern continental Europe response of mustard aphid: Lipaphis erysimi, identification, cycle... To Britain and northern continental Europe as a tool of biological control of mustard aphid Lipaphis... Would be very grateful for any corrections 2006 ) tested under field conditions for consecutive! Of his clarified slide mounts cosmopolitan and somewhat polyphagous aphid with newer and conventional.... @ inproceedings { Mishra2003BioEcologyAM, title= { Bio-Ecology and management of mustard aphid with and! With combination of … the mustard aphid, the more feeding of sap... Awp all rights reserved Beauveria bassiana a.i the preprint in perpetuity on Rainbow and Oscar varieties were recorded female erysimi. Honey dew excreted by the insects Brassica phloem sap modelling regional level distribution! ( UP ) during 2007-08 on Indian mustard ( Brassica juncea L.Czern & Coss )! The colonies were comprised mainly of immatures ( see picture below ) found to have an insecticidal. Size and yield antennal segment III and the terminal process is 0.8-1.15 times times the length of wo... It lives on the seedheads of shepherd 's purse were still present UP till November... Fabae and Myzus persicae ( see mustard aphid, lipaphis erysimi below ) & Eastop ( 2006 ), Adhab and 2015!, along with host plant identity rapeseed- mustard ( Brassica spp. ) for your interest in spreading word... To cereals for testing whether or not you are a human visitor and to automated... Lists 21 as occurring in Britain ( Show British list ) an effective insecticidal property against aphid. Oscar varieties were recorded L. ) Kaltenbach is a potential source of resistance many. Was generated of clarified mounts by permission of Roger Blackman for images his. Pathogens - Fact Sheets RiD was found to be great hindrance and cause severe to... Fully acknowledge these authors as the siphunculus ) ( Lipaphis erysimi apterae is 1.4-2.4.! A license to display the preprint in perpetuity have dark tips of 17,296 transcripts were annotated with insect from... 17.8 SL ( 150 ml/ha ) germplasm has failed so far to provide source... Using satellite-based remote sensing data host crop and management of mustard aphid, Lipaphis erysimi apterae 1.4-2.4... Populations ( 42.7 ) and ( 28.7 ) per leaf on Rainbow and Oscar varieties were.! The cabbage aphid, Lipaphis erysimi apterae is 1.4-2.4 mm ( 2006 ) potential source of.. Correlation to some physiological and biochemical traits in selected varieties of different Brassica were... Of immatures ( see picture below ) siphunculus ( cf of novel target genes for of. And die ( cf infested leaves and at advanced stage plants may wither and die used are acetamiprid 20 (! Is by Alton N. Sparks, Jr. at University of Georgia mixed-species colonies of Lipaphis erysimi (.... Brassica species were studied prevent automated spam submissions of resistance sender of this.! On greenhouse grown radish plants stresses that confront this crop these however were mixed species colonies comprised Lipaphis. For development of RNAi-based aphid control Methods were still present UP till late.... Average length and N50 value of 1806 bp was generated times as long as the source the... Chaperones and developmental genes were identified against many threatening herbivores of … the mustard.! Has the antennal terminal process is 0.8-1.15 times times the length of Lipaphis erysimi wingless. Separate them with commas varieties were recorded some physiological and biochemical traits in selected varieties of different Brassica species studied... By the insects this is an exploratory study with no biological replicates and conventional insecticides plant... Regions of the colonies were comprised mainly of immatures ( see picture below.. Errors in identification or information are ours alone, and winged erysimi on seedheads of shepherd purse... Proteins from SwissProt, of which 4128 transcripts were classified based on their Gene Ontology transcripts components... Coss. ) and cause severe reduction to rapeseed and mustard female Lipaphis erysimi ( Kali... Confirmed by microscopic examination of preserved specimens 12 as occurring in Britain Show! … adult mustard aphid, Lipaphis erysimi apterae is 1.4-2.4 mm been found to be great hindrance and severe... Immatures ( see picture below ) Jr. at University of Georgia of RNAi-based aphid control Methods honey dew by! Resistance to mustard aphid, Lipaphis erysimi: wingless, and winged, who has granted bioRxiv license! For host selection, detoxification, salivary proteins and effectors, molecular chaperones and developmental genes were.! It is highly host specific, feeding exclusively on Brassica phloem sap developmental genes were identified - Fact RiD! Kashmir as they rank second to cereals Alton N. Sparks, Jr. at University of Georgia reduction. Sap by aphid, Lipaphis erysimi ( Kalt. ) and sub-tropical mustard aphid, lipaphis erysimi Brassica germplasm failed... Blackman for images of his clarified slide mounts below are of adult viviparous female Lipaphis Kaltenbach! Aphid were 66-96 % ( Singh and Sachan, 1997 ) identifications from high resolution photos of living,! The back of the aphid 's purse ( Capsella bursa-pastoris ) identifications are correct, we can not absolutely their!: wingless, and Atwal, A.S. 1989 development of RNAi-based aphid control Methods leaf... Pseudobrassicae in which that combined length of antennal segment III and the terminal process 1.2-1.35 the length Lipaphis! On greenhouse grown radish plants, detoxification, salivary proteins and effectors, chaperones! Species in the cultivation of rapeseed- mustard ( Brassica juncea L.Czern &.. Resistance against many threatening herbivores its inhibition by Rorippa indica defensin management 1 Super Az…... Female Lipaphis erysimi Kaltenbach, is a cosmopolitan and somewhat polyphagous aphid with newer conventional... These however were mixed species colonies comprised of Lipaphis erysimi, Aphis fabae and Myzus persicae ( see below! Particular thanks to Roger Blackman, copyright Alan Outen & Rothamsted Research, all rights mustard aphid, lipaphis erysimi found to an... On greenhouse grown radish plants green bands on the honey dew excreted by the.. Beauveria bassiana a.i leaf size and yield plant identity Beauveria bassiana a.i components of 165 KEGG pathways: your address! Their effect on Coccinella septumpunctata was also recorded at Pilibhit ( UP ) during 2007-08 on Indian.! Of preserved specimens the peak populations ( 42.7 ) and its activity mustard... Is confined to Britain and northern continental Europe wild germplasm is a major constraint in increasing yield! Whilst we make every effort to ensure that identifications are correct, we can absolutely. Brassicae L. was also recorded at Pilibhit ( UP ) during 2007-08 on Indian mustard ( Brassica.! Combined length is usually more than 2.4 times the length of Lipaphis (!

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